Part:BBa_K4252023:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4252023

We cultured the plasmids transferred into cI857, phlF, R promoter and mCherry at 30℃ ,33℃ ,37℃ and 42℃ . From the fluorescence data corresponding to our temperature gradient, the red fluorescence expression was very weak in the temperature range of 30 ℃ to 34 ℃, indicating that the dimer formed by cI857 inhibited the R promoter at this time, leading to the subsequent weakening of mCherry expression; The strong red fluorescence expression at 37 ℃ to 42 ℃ indicated that cI857 dimer depolymerized and the inhibition of R promoter was relieved, and then mCherry was normally expressed. That is, the temperature sensitive control element cI857 is controlled by temperature

User Reviews

UNIQ6938f37f27cfadd6-partinfo-00000000-QINU UNIQ6938f37f27cfadd6-partinfo-00000001-QINU